This tool was developed by our lab to
analyze LSM image stacks. LSM image stacks
can be loaded and several regions of interest
can be selected and saved for subsequent processing.
The format used to save the data is described
in this document. The software is free to
use for researchers and non-commercial purposes.
There are still some bugs that haven't been
worked out. Please send an email to mmichael
'at' email.arizona.edu to report such bugs
or make suggestions to improve the software.
IAGUI can be downloaded here.
Program Features
- Reads LSM format image stacks using the tiffread() function
created by Francois Nedelec but modified
to read more than 3 channels.
- Calculates ridge orientation using the algorithm and
software developed by Peter Kovesi. The
software was originaly used for fingerprint
ridge orientation/detection.
- Can select a region of interest and calculate the average
orientation and also save the orientation
information in that region for later processing
for a given channel.
- Select a region of interest and find the intensity histogram
and also save the data in that region for
later processing. This is calculated for
all channels regardless of the selected
analysis channel.
- Customize the image view by selecting different colors for
each channel. Also the option to adjust
image contrast, image transparency and other
image properties is provided.
- Adds a 50um bar indicator at the top left corner of the image.
- Can draw a line on the image and get the intensity values for
all channels that fall under the line and
plot the profile.
- Draw a line and determine its angle. This is useful when a
measurement is needed on the orientation
of various structures.
Requirements
- Matlab version 7.4 or later
- Matlab Image Processing Toolbox
- Add the path that contains the IAGUI files to the Matlab
path
- The mwxpccontrolsx2x7x1.ocx module is essential for the
operation of IAGUI and must be registered
with Windows. Use "regsvr32 mwxpccontrolsx2x7x1.ocx"
on the command line to register it. Alternatively
run the install.bat file in the IAGUI folder.
- Minimum screen resolution 1280x1024
Contributors & Contact Info
Stylianos Andreou : stelios 'at' andreou.com
Michalis Michaelides : mmichael 'at' email.arizona.edu
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